A Preclinical Murine Model of Hepatic Metastases

The overall goal of this procedure is to create a preclinical mirroring pancreatic cancer model of hepatic metastases via Hemi spleen technique. This is accomplished by first creating a single cell suspension of cultured pan O2 tumor cells. The second step of the procedure is to express and divide the spleen of a fully anesthetized mouse.

The next steps are to inject tumor cells into a single Hemi spleen and remove the injected hemi spleen. The final step is to close the abdomen and recover the mouse from anesthesia. Ultimately, results can be obtained that show differences in metastatic disease progression by monitoring survival, followed by liver necropsy, and additional non-invasive imaging.