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JoVE Journal
Neuroscience

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Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters
 

Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters

Article DOI: 10.3791/51795
July 8th, 2014

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Summary July 8th, 2014

This article describes a neuronal culture system that can be used to obtain large quantities of pure axonal samples for biochemical and immunocytochemical analyses. This technique will allow an improved understanding of normal axonal physiology and signaling pathways leading to neurodegeneration.

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