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Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters
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Chapters
- 00:05Title
- 01:29Coating of Filters
- 02:49Dissecting Embryonic Dorsal Root Ganglion (DRG) Explants
- 04:08Seeding and Maintenance of Dorsal Root Ganglion Explants on Filters
- 05:23Trophic Factor Withdrawal Treatment
- 06:02Axonal Transection to Induce Wallerian Degeneration
- 06:59Protein Extraction of Axonal Samples
- 08:25Immunofluorescence Examination of Axons on Filters
- 09:42Results: Axonal Preparations and Axonal Degeneration Studies
- 11:24Conclusion
This article describes a neuronal culture system that can be used to obtain large quantities of pure axonal samples for biochemical and immunocytochemical analyses. This technique will allow an improved understanding of normal axonal physiology and signaling pathways leading to neurodegeneration.
