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July 08, 2014
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This article describes a neuronal culture system that can be used to obtain large quantities of pure axonal samples for biochemical and immunocytochemical analyses. This technique will allow an improved understanding of normal axonal physiology and signaling pathways leading to neurodegeneration.
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Cite this Article
Unsain, N., Heard, K. N., Higgins, J. M., Barker, P. A. Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters. J. Vis. Exp. (89), e51795, doi:10.3791/51795 (2014).
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