Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

Mayandi Sivaguru; Glenn A. Fried; Carly A. H. Miller; Bruce W. Fouke

doi:10.3791/51824

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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

12,267 Views

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10:39 min

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September 05, 2014

DOI:

10.3791/51824-v

DOI:

10.3791/51824-v

•

10:39 min

September 05, 2014

•

2 Views

Mayandi Sivaguru¹, Glenn A. Fried¹, Carly A. H. Miller^1,2, Bruce W. Fouke^1,2,3

¹Institute for Genomic Biology,University of Illinois at Urbana-Champaign, ²Department of Geology,University of Illinois at Urbana-Champaign, ³Department of Microbiology,University of Illinois at Urbana-Champaign

Summary

An integrated suite of imaging techniques has been applied to determine polyp morphology and tissue structure in the Caribbean corals Montastraeaannularis and M. faveolata. Fluorescence, serial block face, and two-photon confocal laser scanning microscopy have identified lobate structure, polyp walls, and estimated chromatophore and zooxanthellae densities and distributions.