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A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
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1Department of Biomedical Engineering,National Taiwan University, 2Institute of Pharmacology, College of Medicine,National Taiwan University, 3Molecular Imaging Center,National Taiwan University, 4Department of Dermatology,National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine,National Taiwan University, 6Department of Ophthalmology,Chang Gung Memorial Hospital, 7College of Medicine,Chang Gung University
Chapters
- 00:04Introduction
- 00:40Multiphoton Microscopy Setup
- 01:29Eye Holder Setup
- 02:38Z-Serial Image Acquisition
- 03:43Image Processing and 3D Reconstruction
- 04:36Results: Representative Mouse Cornea and Conjunctiva Imaging
- 06:10Conclusion
Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures.
