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JoVE Journal
Bioengineering

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A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
 

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Article DOI: 10.3791/60944
May 17th, 2020

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Summary May 17th, 2020

Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures.

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