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JoVE Journal
Immunology and Infection

This content is Open Access.

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
 

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Article DOI: 10.3791/51960
September 2nd, 2014

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Summary September 2nd, 2014

This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA by electroporation with high transfection efficiency while maintaining high cell vitality and full macrophage capacity for differentiation and polarization.

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