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Transfecting Human Neural Stem Cells with the Amaxa Nucleofector

Article DOI: 10.3791/240
July 19th, 2007 •
1, 1
1Department of Pathology, University of California, Irvine (UCI)

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July 19th, 2007

Introducing a gene of interest into a cell is a powerful method for elucidating its function in vivo. This protocol describes an efficient method of transfecting a culture of human neural stem/precursor cells (hNSPCs) using the Nucleofector electroporation apparatus made by Amaxa.

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