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Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

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06:46 min

November 09, 2019

DOI:

06:46 min
November 09, 2019

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Summary

Automatically generated

Macrophages, especially primary macrophages, are challenging to transfect as they specialize in detecting molecules of non-self origin. We describe a protocol that allows highly efficient transfection of primary macrophages with mRNA generated from DNA templates such as plasmids.

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