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JoVE Journal
Immunology and Infection

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Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
 

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

Article DOI: 10.3791/60143-v
November 9th, 2019

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Summary November 9th, 2019

Macrophages, especially primary macrophages, are challenging to transfect as they specialize in detecting molecules of non-self origin. We describe a protocol that allows highly efficient transfection of primary macrophages with mRNA generated from DNA templates such as plasmids.

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