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JoVE Journal Neuroscience
Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Article DOI: 10.3791/52241-v • 13:05 min • December 18th, 2014
December 18th, 2014 •
1, 1,2, 1,3, 1, 1,4
1Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, 2Spemann Graduate School of Biology and Medicine and Faculty of Biology, University of Freiburg, 3School of Life Sciences, Keele University, 4Center for Biological Signaling Studies (BIOSS), University of Freiburg

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We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.

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Flow Cytometry Surface Antigen Analysis Intracellular Antigen Analysis Neural Cell Types Protocols Cluster Of Differentiation (CD) Flow Cytometric Analysis Carboxyfluorescein Succinimidyl Ester (CFSE) Surface Antigen Staining CD Antibodies Intracellular Antigen Detection Primary Antibodies Secondary Antibodies Fluorescently Labeled Fab Fragments Zenon Labeling Video Demonstration Experimental Planning Critical Controls Flow Cytometric Analysis Principles Gating Strategy Compensation For Spectral Overlap Neurobiologists
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