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Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

DOI:
10.3791/61050-v

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09:01 min

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November 17, 2020

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, , , ,
1Translational Medicine Research Center, Ruijin Hospital North,Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology,Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation,Shanghai Jiao Tong University School of Medicine

Chapters

  • 00:04Introduction
  • 00:53C57BL/6 Mouse Immunization
  • 01:58Single Brain Cell Suspension Preparation
  • 04:19Flow Cytometric Analysis
  • 07:00Results: Representative Experimental Autoimmune Encephalomyelitis (EAE) Course and Brain Lymphocyte Analyses
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript presents a protocol to induce active experimental autoimmune encephalomyelitis (EAE) in mice. A method for the isolation and characterization of the infiltrated lymphocytes in the central nervous system (CNS) is also presented to show how lymphocytes are involved in the development of CNS autoimmune disease.

Tags

Keywords: Flow CytometryLymphocyte InfiltrationCentral Nervous SystemExperimental Autoimmune EncephalomyelitisImmune CellsMOG 35-55Pertussis ToxinNeurological Signs

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