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Developmental Biology

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JoVE Journal Developmental Biology
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
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Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Article DOI: 10.3791/52410-v • 10:48 min • April 12th, 2015
April 12th, 2015 •
1, 1, 1
1ReNeuron

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With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.

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Differentiation Human Neural Stem Cell Line Three Dimensional Cultures MicroRNA Putative Target Genes NSCs Self-renewal Neurons Astrocytes Oligodendrocytes Local Microenvironments Clinical Trials Stroke Disability Ethical Approved First Trimester Human Fetal Cortex Retroviral Integration C-mycERTAM Construct Axon Process Outgrowth 3D Scaffolds MiRNA Expression PCR Array Analysis Computational Analysis MiRNA Putative Targets SOX5 NR4A3 Down-regulated MiRNAs MiRNA Target Validation Dual Reporter Plasmid Transfection Dual Luciferase Assay
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