Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

Kate L. Erickson; Michelle J. Hickey; Yuki Kato; Colin C. Malone; Geoffrey C. Owens; Robert M. Prins; Linda M. Liau; Noriyuki Kasahara; Carol A. Kruse

doi:10.3791/52416

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Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

7,748 Views

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10:01 min

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February 11, 2015

DOI:

10.3791/52416-v

DOI:

10.3791/52416-v

•

10:01 min

February 11, 2015

•

7726 Views

Kate L. Erickson*¹, Michelle J. Hickey*¹, Yuki Kato², Colin C. Malone¹, Geoffrey C. Owens¹, Robert M. Prins^1,2, Linda M. Liau^1,4,5, Noriyuki Kasahara^3,5, Carol A. Kruse^1,4,5

¹Department of Neurosurgery,UCLA David Geffen School of Medicine ²Department of Molecular and Medical Pharmacology,UCLA David Geffen School of Medicine ³Department of Medicine,UCLA David Geffen School of Medicine ⁴Brain Research Institute,UCLA David Geffen School of Medicine ⁵Jonsson Comprehensive Cancer Center,UCLA David Geffen School of Medicine

Summary

We describe a protocol to monitor radial mobility of non-adherent immune cells in vitro using a cell sedimentation manifold/slide apparatus. Cell migration is tracked on monolayers of tumor cells or on extracellular matrix proteins. Examination by light and fluorescence microscopy allows for observation of cell mobility and cytotoxic functionality.