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Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo

Article DOI: 10.3791/53290-v • 12:48 min • May 12th, 2016
May 12th, 2016 •
1, 2, 1, 2,3, 1
1Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 2Department of Physics, University of California, San Diego, 3Section of Neurobiology, University of California, San Diego

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Summary
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We present a protocol to create cell-based neurotransmitter fluorescent engineered reporters (CNiFERs) for the optical detection of volumetric neurotransmitter release.

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CNiFERs Cell-based Neurotransmitter Fluorescent Reporters Optical Detection Neurotransmitters In Vivo Methodology Construction Testing Neuromodulators Timing Release GPCRs HEK293 Cells Calcium Detector Chimeric G Protein T-25 Flask Incubator Lenta Virus Media Mixture Trypsin Growth Media Freezing Storage
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