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Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

DOI:
10.3791/53676-v

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12:52 min

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November 28, 2015

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, ,
1Department of Neurosurgery,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan

Chapters

  • 00:05Title
  • 00:46Stereotactic Engraftment of Glioma Cells into Mouse Striatum
  • 02:52Mouse Transcardial Perfusion and Brain Harvesting
  • 04:31Isolation of Glioma-infiltrating PBMCs
  • 09:40Results: Flow Cytometric Gating Strategy Used to Identify Glioma-infiltrating Gr-1+/ CD11b+ Myeloid Cells and NK.1 + Cells
  • 11:27Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a straightforward method for the isolation and flow cytometric analysis of glioma-infiltrating peripheral blood mononuclear cells that yields time-dependent quantitative data on the number and activation status of immune cells entering the early brain tumor microenvironment.

Tags

IsolationFlow Cytometric AnalysisGlioma-infiltratingPeripheral Blood Mononuclear CellsNK CellsGalectin-1Myeloid CellsTumor RejectionShRNA KnockdownGL26 GliomasBrain Tumor MicroenvironmentSyngeneic C57BL/6J Mouse BrainFlow Cytometric Analysis

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