An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
August 14th, 2016
An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.
The overall goal of this procedure is to establish a mouse spontaneous metastasis breast cancer model that allows the quantification of primary tumor breast cancer growth as well as the visualization and measurement of the breast cancer metastasis kinetics.This method can help answer key questions in the field of spontaneous cancer metastasis, such as how can altering tumor-associated genes affect cancer growth and metastasis?The main advantage of this technique is that this model mimics human breast cancer development, making it ideal for testing the efficacy of therapeutic agents.On the day of the experiment, harvest an overnight culture of 4T1 luciferase tumor cells, and pellet the cells by centrifugation.Remove the supernatant by vacuum, then wash the cells in 10ml of PBS, and re-suspend the pellet in 10ml of fresh PBS for counting.Next, dilute the cells to 2x10^5 cells/ml concentration in HBSS, and output the suspension into 1.5ml sterile microcentrifuge tubes on ice.Then, restraining the mouse by hand, label the mammary glands of a 6-8 week-old female BALB/c mouse with a black marker, and use an electronic hair trimmer to shave the hairs surrounding the number 3 nipple.Proceed to re-suspend the cells by 2-3 inversions of the microfuge tube, then carefully aspirate 50
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Overview
This study presents an orthotopic breast cancer primary tumor model that allows for the surgical removal of the primary tumor to extend the lifespan of mice. The model facilitates the generation of spontaneous metastasis, with tumor growth and progression monitored through luciferase fluorescence imaging.
Key Study Components
Area of Science
- Oncology
- Animal Models
- Metastasis Research
Background
- Breast cancer is a leading cause of cancer-related deaths.
- Understanding metastasis is crucial for developing effective treatments.
- Animal models are essential for studying cancer progression.
- Luciferase imaging provides a non-invasive method to monitor tumor dynamics.
Purpose of Study
- To develop a model for studying breast cancer metastasis.
- To evaluate the effects of primary tumor removal on mouse lifespan.
- To utilize imaging techniques for monitoring tumor growth.
Methods Used
- Orthotopic implantation of breast cancer cells in mice.
- Surgical removal of the primary tumor.
- Luciferase fluorescence imaging for tumor monitoring.
- Statistical analysis of survival data.
Main Results
- Successful establishment of a breast cancer model in mice.
- Extended lifespan observed post-surgical removal of the primary tumor.
- Quantitative data on tumor growth and metastasis progression.
- Effective use of luciferase imaging for real-time monitoring.
Conclusions
- The model is valuable for studying breast cancer metastasis.
- Surgical intervention can prolong lifespan in this model.
- Imaging techniques enhance the understanding of tumor dynamics.
Frequently Asked Questions
Chapters in this video
0:05
Title
0:48
Orthotopic Breast Cancer Tumor Establishment
2:17
Live Mouse Bioluminescent Tumor Growth Imaging
3:24
Surgical Primary Tumor Removal
4:36
Lung Metastases Validation
6:13
Results: Representative Evaluation of Spontaneous Breast Cancer Metastasis
7:00
Conclusion
