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Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs
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JoVE Journal Medicine
Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs

Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs

DOI:
10.3791/54077-v

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10:47 min

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July 02, 2016

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, , , , , , ,
1College of Veterinary Medicine,Gyeongsang National University, 2PWG Genetics Pte Ltd.

Chapters

  • 00:05Title
  • 01:39Collecting Bone Marrow from Minipigs
  • 02:47Isolation of Cells and In Vitro Cultivation
  • 04:42In Vitro Cultivation of Cells Isolated from Bone Marrow
  • 06:18Collecting Synovial Fluid and Cultivating Isolated Cells
  • 08:19Results: Bone Marrow- and Synovial Fluid-derived MSC Morphology and Cell Surface Marker Expression
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.

Tags

Keywords: Mesenchymal Stem CellsSynovial FluidBone MarrowMinipigsRegenerationImmunomodulationJoint DiseasesAutologousBone Marrow ExtractionDensity CentrifugationMononuclear Cells

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