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A Method for Microinjection of Patiria minata Zygotes
 

A Method for Microinjection of Patiria minata Zygotes

Article DOI: 10.3791/51913
September 1st, 2014

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Summary September 1st, 2014

Methods that produce morphant embryos are essential to study developmental mechanisms and gene regulatory networks. The sea star Patiria miniata is an emerging model system for these studies. Here we present a protocol for obtaining gametes, producing cultures of embryos, and rapid microinjection of zygotes from this species.

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