This video demonstrates how to visualize axonal pathways of genetically defined groups of neurons in the embryonic chick spinal cord utilizing in ovo electroporation of reporter genes under the control of specific enhancer elements.
Avraham, O., Zisman, S., Hadas, Y., Vald, L., Klar, A. Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation. J. Vis. Exp. (39), e1792, doi:10.3791/1792 (2010).