Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing <em>in ovo</em> Electroporation

Oshri Avraham; Sophie Zisman; Yoav Hadas; Lilach Vald; Avihu Klar

doi:10.3791/1792

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Uti...

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Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

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9,664 Views

07:55 min

May 2, 2010

DOI: 10.3791/1792-v

Oshri Avraham*¹, Sophie Zisman*¹, Yoav Hadas¹, Lilach Vald¹, Avihu Klar¹

¹Department of Medical Neurobiology,Institute for Medical Research Israel Canada, Hebrew University-Hadassah Medical School

Overview

This video demonstrates a procedure for visualizing axonal pathways of genetically defined neurons in the embryonic chick spinal cord using in ovo electroporation. The method allows researchers to study axonal trajectories effectively.

Key Study Components

Area of Science

Neuroscience
Developmental Biology
Electrophysiology

Background

Understanding axonal pathways is crucial for neuroscience research.
In ovo electroporation is a technique used to introduce reporter genes.
The embryonic chick spinal cord serves as a model for studying neuronal development.
Genetically defined neuronal populations can provide insights into specific neural circuits.

Purpose of Study

To visualize axonal pathways in the embryonic spinal cord.
To utilize in ovo electroporation for gene expression studies.
To enhance understanding of neuronal development and connectivity.

Methods Used

Transfer fertilized chicken eggs to a humidified incubator.
Maintain incubation at 37 to 38 degrees Celsius.
Perform in ovo electroporation to introduce reporter genes.
Prepare spinal cord open book preparations for visualization.

Main Results

Successful visualization of axonal pathways in the spinal cord.
Demonstration of the effectiveness of in ovo electroporation.
Insights into the trajectories of genetically defined neuronal populations.
Establishment of a reliable method for studying neuronal development.

Conclusions

The method provides a valuable tool for neuroscience research.
In ovo electroporation is effective for gene delivery in embryonic models.
Understanding axonal pathways can inform studies on neural circuit formation.

Frequently Asked Questions

What is in ovo electroporation?

In ovo electroporation is a technique used to introduce DNA into embryonic tissues by applying an electric field.

Why use the embryonic chick spinal cord?

The embryonic chick spinal cord is a well-established model for studying neuronal development and connectivity.

What are reporter genes?

Reporter genes are genes that researchers use to study gene expression and track cellular processes.

How does this method help in neuroscience?

This method allows for the visualization of specific neuronal pathways, enhancing our understanding of neural circuits.

What temperature is optimal for incubating chicken eggs?

The optimal incubation temperature for fertilized chicken eggs is between 37 to 38 degrees Celsius.

What is the significance of studying axonal trajectories?

Studying axonal trajectories helps researchers understand how neurons connect and communicate within the nervous system.

This video demonstrates how to visualize axonal pathways of genetically defined groups of neurons in the embryonic chick spinal cord utilizing in ovo electroporation of reporter genes under the control of specific enhancer elements.

Hi, I am Ham from the laboratory of Dr.Review Klar at the medical neurobiology department in the Hebrew University of Jerusalem. Hi, I'm Sophie Esman and I'm also from a Lars lab. Today we are going to show you a procedure for Innovo, electroporation and spinal cord open book preparation.

We use this procedure in our laboratory to study axonal trajectories of genetically defined neuronal population in the embryonic spinal cord. So let's get started. To begin this procedure, transfer fertilized white leghorn chicken eggs from room temperature to a humidified incubator, preferably with rocking trays at 37 to 38 degrees Celsius.

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