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Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
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Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

DOI:
10.3791/55031-v

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10:57 min

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February 03, 2017

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, , , ,
1Biodiversity and Conservation Department,Real Jardín Botánico, CSIC, 2Research Support Unit,Real Jardín Botánico, CSIC, 3Mycology Department,Real Jardín Botánico, CSIC, 4Division of Glycoscience, AlbaNova University Center,Royal Institute of Technology (KTH)

Chapters

  • 00:05Title
  • 00:57Collection and Fixation of Developing and Fully Formed Plant Structures
  • 02:04Dissection and Dehydration of Developing and Fully Formed Plant Structures
  • 03:02Mounting and Preparing Plant Tissues for SEM Observation of Developing and Fully Formed Plant Structures
  • 04:20Study of Cyst Behavior of Saprolegnia (Oomycetes) on Different Surfaces
  • 06:04Study of Herbarium Fungal Spores of Phellorinia herculanea under SEM
  • 07:20Results: Recovery of Plant Development, Differential Spine Growth of Saprolegnia parasitica, and Nurse Cells in Spores of Phellorinia herculanea f. stellata
  • 09:01Conclusion

Summary

Automatic Translation

Automatic Translation

Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.

Tags

Keywords: Scanning Electron Microscopy (SEM)SEM ProtocolsPlant SamplesOomycete SamplesFungal SamplesFixationFAA Fixative70% EthanolFormaldehydeAcetic AcidDehydrationCritical Point DryingSample MountingSEM Sample Holders

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