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Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds
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JoVE Journal Biology
Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

DOI:
10.3791/63777-v

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11:48 min

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May 17, 2022

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, ,
1LabPlaM – Mycoheterotrophic Plants Research Lab, Department of Plant Biology,University of Campinas (UNICAMP)

Chapters

  • 00:05Introduction
  • 00:50Collecting, Fixing, and Maintaining Samples
  • 02:34Surface Analysis of Organs in Fixed and Non-Fixed Material
  • 03:18Application of a Fluorochrome Conjugated to Wheat Germ Agglutinin in Fluorescence and Confocal Microscopy
  • 04:14Scanning Electron Microscopy of Plant Organs
  • 07:30Symbiotic Germination of Orchid Seeds
  • 09:41Results: Study of Fungal Colonization in Plant Tissues and Seeds Germinated with Mycorrhizal Fungi
  • 11:13Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol aims to provide detailed procedures for collecting, fixing, and maintaining mycoheterotrophic plant samples, applying different microscopy techniques such as scanning and transmission electron microscopy, light, confocal, and fluorescence microscopy to study fungal colonization in plants tissues and seeds germinated with mycorrhizal fungi.

Tags

Keywords: Microscopy TechniquesFungal ColonizationMycoheterotrophic PlantsSymbiotic GerminationSeedsStructural BotanyMycorrhizal InteractionsPhysiologyReproductive BiologyEvolutionEcologyTransmission Electron MicroscopyFixationDissecting MicroscopeRhizomorphs

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