Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description

Laura Greimers; Pierre V. Drion; Alain Colige; Vincent Libertiaux; Vincent Deno&#235;l; Christelle Lecut; Andr&#233; Gothot; Jean-Fran&#231;ois Kaux

doi:10.3791/55759

Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Ten...

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description

Full Text

9,769 Views

07:51 min

March 19, 2018

DOI: 10.3791/55759-v

Laura Greimers¹, Pierre V. Drion¹, Alain Colige², Vincent Libertiaux³, Vincent Denoël³, Christelle Lecut⁴, André Gothot⁴, Jean-François Kaux⁵

¹Experimental Surgery, GIGA-R & Credec,University of Liège, ²Laboratory of Connective Tissues Biology, GIGA-R,University of Liège, ³Department Argenco,University of Liège, ⁴Department of Clinical Biology, University Hospital of Liège,University of Liège, ⁵Physical Medicine and Sport Traumatology Department, FIFA Medical Center of Excellence, University Hospital of Liège,University of Liège

Overview

This protocol describes the evaluation of tendon healing in rats injected with platelet-rich plasma (PRP) or saline after Achilles tendon surgery. The healing process is assessed at various time points using standardized analyses.

Key Study Components

Area of Science

Neuroscience
Biology
Musculoskeletal Research

Background

Platelet-rich plasma (PRP) is used to enhance healing in musculoskeletal injuries.
The study focuses on the similarities in tendon healing between rats and humans.
Standardized surgical and biomechanical procedures are crucial for reproducibility.
Insights gained can inform PRP applications in various medical fields.

Purpose of Study

To observe the effects of PRP on healing in surgically-sectioned rat Achilles tendons.
To provide a method for evaluating tendon lesions in a controlled environment.
To explore the implications of PRP treatment for human tendon injuries.

Methods Used

Injection of PRP or saline into rat Achilles tendons.
Assessment of tendon healing at multiple time points.
Standardized surgical procedures to ensure consistency.
Biomechanical analyses to evaluate tendon resistance and healing.

Main Results

PRP treatment showed significant effects on tendon healing compared to saline.
Standardized methods allowed for reliable assessment of healing progress.
Results may inform future PRP applications in human medicine.
Visual demonstrations of the method enhanced understanding of the procedure.

Conclusions

PRP is a promising treatment for enhancing tendon healing.
Findings support the use of animal models for studying human tendon injuries.
Standardized protocols are essential for reproducible results.

Frequently Asked Questions

What is the role of PRP in tendon healing?

PRP is used to enhance the healing process of tendons after surgical intervention.

How does this study relate to human medicine?

The study's findings on tendon healing in rats can inform PRP applications in treating human tendon injuries.

What are the key components of the methodology?

The methodology includes standardized surgical procedures, PRP injection, and biomechanical assessments.

Why is visual demonstration important in this study?

Visual demonstrations help ensure that surgical and biomechanical steps are consistently reproducible.

What are the implications of this research?

The research may lead to improved treatments for tendon injuries in both animals and humans.

This protocol describes the evaluation process of healing tendons in rats that have been injected with allogeneic platelet rich plasma (PRP) or saline solution after removing part of the Achilles tendon. The progress of tendon healing is evaluated at several time points using different types of analyses.

The overall goal of this procedure is to observe the effects of a single injection of Platelet-Rich Plasma, or PRP, on the resistance and healing process of surgically-sectioned rat Achilles tendons at various time points. This method can help answer quick questions on musculoskeletal field about tendon lesions. The main advantage of this technique is that the surgical, biomechanical, and PRP progression procedures are well-standardized.

The implications of these techniques extend toward the use of PRP in humans, because these types of lesion and the tendon healing process are similar between rats and human. Though this method can provide insight into PRP preparation for musculoskeletal injuries, this injection technique can also be applied to other systems, such as heart or skin disease. Visual demonstration of the method is very important, because surgical and biomechanical steps require consistent reproducibility in order to obtain optimal results.

View the full transcript and gain access to thousands of scientific videos