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JoVE Journal
Developmental Biology

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Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
 

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Article DOI: 10.3791/54901
November 29th, 2016

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Summary November 29th, 2016

We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.

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