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Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

DOI:
10.3791/54901-v

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08:40 min

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November 29, 2016

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1Program in Developmental Biology,Baylor College of Medicine, 2Department of Genetics,UT MD Anderson Cancer Center

Chapters

  • 00:05Title
  • 00:35Dissection of Adult Worms for Obtaining Gonads
  • 02:21Gonad Fixation
  • 04:22Blocking and Antibody-staining
  • 06:18Mounting the Tissue
  • 07:15Results: ERK Activity in Germline Tissues
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.

Tags

Keywords: ERK SignalingC. ElegansGermlineIn VivoQuantificationSpatial And Temporal AnalysisDrug TreatmentsGene PerturbationsDissectionImmunofluorescenceMicroscopy

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