Journal
/
Developmental Biology
/
Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

DOI:
10.3791/55794-v

•

10:16 min

•

June 14, 2017

•

, , , ,
1School of Biomedical Sciences,The University of Hong Kong, 2Department of Orthopaedics and Traumatology,The University of Hong Kong

Chapters

  • 00:05Title
  • 00:47MSC Culture Establishment and Expansion
  • 02:13Schwann Cell-like Cell (SCLC) Generation
  • 04:40Dorsal Root Ganglion (DRG) Purification and SCLC Co-culture
  • 07:44Results: Representative Schwann Cell Generation
  • 09:08Conclusion

Summary

Automatic Translation

Automatic Translation

Marrow stromal cells (MSCs) with neural potential exist within the bone marrow. Our protocol enriches this population of cells via hypoxic preconditioning and thereafter directs them to become mature Schwann cells.

Tags

Keywords: Hypoxic PreconditioningMarrow-derived Progenitor CellsSchwann CellsStem CellRegenerative MedicineNeural ProgenitorsBone Marrow Stromal CellsAutologous Cell TherapyMSC Growth MediumCell DissociationHypoxia ChamberHepesNitrogenOxygen

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55794?article_type=v

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
check_url/55794?article_type=v

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
check_url/55794?article_type=v

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
check_url/55794?article_type=v

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
check_url/55794?article_type=v

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
check_url/55794?article_type=v

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
check_url/55794?article_type=v

Single-cell Photoconversion in Living Intact Zebrafish
check_url/55794?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
check_url/55794?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
check_url/55794?article_type=v

In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells

Read Article