Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
December 16th, 2017
A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.
The overall goal of this procedure is to perform continuous live imaging and single cell tracking of multiple neural cell populations.Followed by post-imaging immunocytochemistry, this method allows the analysis of any cellular events that take place within the track time period.This method can help to answer key questions in the neuroscience field, such as cell behavior and lineage progression of multiple neural population as well as the identification of their modulators.The main advantage of live imaging and single cell tracking in real time is that it allows to monitor variations in cell biology that may pass unnoticed in approaches based on end point analysis.This demonstration will guide the researchers through main steps that can be easily adapted to any culture condition and acquisition software.My name is Rosa Gomez, and I'll be demonstrating the plating and post-imaging immunocytochemistry steps.My name is David Agust
Overview
This article describes a robust protocol for monitoring neural populations through time-lapse video microscopy and software-based post-processing. This method enables the identification of biological events in selected populations during live imaging experiments.
Key Study Components
Area of Science
- Neuroscience
- Cell Biology
- Live Imaging Techniques
Background
- Continuous live imaging allows for real-time observation of cellular events.
- Single cell tracking provides insights into cell behavior and lineage progression.
- Post-imaging immunocytochemistry enhances the analysis of cellular events.
- This approach can reveal variations in cell biology that may be missed in endpoint analyses.
Purpose of Study
- To monitor variations in cell biology in neural populations.
- To identify modulators of cell behavior.
- To provide a protocol adaptable to various culture conditions and acquisition software.
Methods Used
- Time-lapse video microscopy for live imaging.
- Single cell tracking of multiple neural populations.
- Post-imaging immunocytochemistry for detailed analysis.
- Guidance through main procedural steps for researchers.
Main Results
- Successful tracking of multiple neural cell populations.
- Identification of key cellular events during live imaging.
- Demonstration of the method's adaptability to different conditions.
- Insights into cell behavior and lineage progression.
Conclusions
- This method is a powerful tool for neuroscience research.
- Real-time monitoring can uncover important biological variations.
- Post-processing techniques enhance the understanding of cellular dynamics.
Frequently Asked Questions
Chapters in this video
0:05
Title
1:14
Live Imaging by Time-lapse Video-microscopy
4:02
Post-imaging Immunocytochemistry, Data Collection, and Processing
6:38
Single-cell Tracking
8:56
Results: Example of aNSCs Isolated from the SEZ and Analyzed by Live Imaging and Single Cell Tracking
10:17
Conclusion
