Journal
/
Developmental Biology
/
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

DOI:
10.3791/56768-v

•

10:25 min

•

January 23, 2018

•

, , , ,
1Institute of Reconstructive Neurobiology,University of Bonn, 2Central Institute of Mental Health,University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)

Chapters

  • 00:05Title
  • 00:55iPSC Aggregate Generation
  • 02:30Anterior Neuroectoderm Induction
  • 03:51Neuroectodermal Aggregates embedding within a Matrix Scaffold
  • 05:49Forebrain-type Organoid Generation, Fixation, and Validation
  • 07:58Results: Representative Forebrain-type Organoid Validation
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

Cerebral organoids represent a new model system to investigate early human brain development in vitro. This article provides the detailed methodology to efficiently generate homogeneous dorsal forebrain-type organoids from human induced pluripotent stem cells including critical characterization and validation steps.

Tags

Keywords: Human Induced Pluripotent Stem CellsForebrain-type Cerebral OrganoidsStandardized And Reproducible Organoid GenerationModeling NeurodevelopmentCortical GenesisAggregationAnterior Neuroectoderm Induction

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56768?article_type=v

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
check_url/56768?article_type=v

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
check_url/56768?article_type=v

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
check_url/56768?article_type=v

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
check_url/56768?article_type=v

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
check_url/56768?article_type=v

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
check_url/56768?article_type=v

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/56768?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
check_url/56768?article_type=v

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
check_url/56768?article_type=v

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Read Article