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Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

DOI:
10.3791/63714-v

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05:40 min

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May 05, 2022

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, ,
1Australian Institute for Bioengineering and Nanotechnology,The University of Queensland

Chapters

  • 00:04Introduction
  • 00:49Cortical Brain Organoids Generation
  • 02:33Characterization of Neuronal Aging in Cortical Organoids
  • 03:20Results: Neuroectodermal Differentiation and Characterization of Cortical Brain Organoids and Their Senescence
  • 04:59Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, we provide a detailed technique for a simple yet robust cortical organoid culture system using standard feeder-free hPSC cultures. This is a rapid, efficient, and reproducible protocol for generating organoids that model aspects of brain senescence in vitro.

Tags

Keywords: Brain OrganoidsCortical Brain OrganoidsNeuronal SenescenceHPSCsNeuroectodermal ColoniesNeuro Epithelial SpheroidsIn Vitro CultureAgingNeuronal ProcessesNeuroectodermal InductionDispaseBFGFCryosectioningBeta-galactosidase

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