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Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts
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Cancer Research
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JoVE Journal Cancer Research
Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts

Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts

DOI:
10.3791/57558-v

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07:50 min

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May 25, 2018

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, , , , , , , ,
1Departments of Surgery, C.S Mott Children’s and Women’s Hospital,The University of Michigan Medical School, 2Departments of Pathology, C.S Mott Children’s and Women’s Hospital,The University of Michigan Medical School, 3Unit for Laboratory Animal Medicine,The University of Michigan Medical School, 4Departments of Radiology, C.S Mott Children’s and Women’s Hospital,The University of Michigan Medical School, 5Departments of Pediatrics, C.S Mott Children’s and Women’s Hospital,The University of Michigan Medical School

Chapters

  • 00:04Title
  • 01:13Preparation of Primary Patient-derived Tumor Cells
  • 03:29Ultrasound Guided Implantation
  • 05:48Results: In Vivo Ultrasound Imaging of Tumor Engraftment and Growth
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to utilize ultrasound-guided injection of neuroblastoma (NB) and Ewing's sarcoma (ES) cells (established cell lines and patient-derived tumor cells) at biologically relevant sites to create reliable preclinical models for cancer research.

Tags

Keywords: Ultrasound-guidedCellular ImplantationMetastatic Tumor XenograftsPatient-derivedNeuroblastomaOrthotopic XenograftCancer BiologyTranslational ResearchTumor EvolutionTherapeutic ResponsesMetastasesPreclinical StudiesDrug DiscoveryTumor DissociationCell SuspensionCell StrainerHemocytometer

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