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Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
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Immunology and Infection
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JoVE Journal Immunology and Infection
Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

DOI:
10.3791/57574-v

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10:45 min

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June 13, 2018

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, , , ,
1Department of Biotechnology and Biosciences,University of Milano-Bicocca, 2Harvard Medical School and Division of Gastroenterology,Boston Children’s Hospital

Chapters

  • 00:05Title
  • 00:54C. albicans Preparation
  • 02:29Deep Dermal Injection
  • 03:57Sample Collection and Preparation for Histological Staining and Slides
  • 05:09Hematoxin & Eosin Staining
  • 06:07Periodic Acid-Schiff Staining
  • 07:19Sample Collection and Preparation for RNA Extraction
  • 08:30Results: Histological Analysis of C. albicans Infection in Mice
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol that allows histological and molecular analysis of skin samples after Candida albicans intradermal injection. This protocol maintains the structural integrity of the skin and allows for the localization of tissue-resident or newly recruited immune cells as well as the pathogen distribution.

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