/
/
Dyb Dermal injektion som en Model af Candida albicans hudinfektion histologisk analyser
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:54C. albicans Preparation
- 02:29Deep Dermal Injection
- 03:57Sample Collection and Preparation for Histological Staining and Slides
- 05:09Hematoxin & Eosin Staining
- 06:07Periodic Acid-Schiff Staining
- 07:19Sample Collection and Preparation for RNA Extraction
- 08:30Results: Histological Analysis of C. albicans Infection in Mice
- 10:00Conclusion
Her beskriver vi en protokol, der giver mulighed for histologiske og molekylær analyse af hud prøver efter Candida albicans intradermal injektion. Denne protokol fastholder den strukturelle integritet af huden og giver mulighed for lokalisering af væv-resident eller nyligt rekrutterede immunceller samt patogen distribution.
