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In Vitro Canine neutrofile ekstracellulære fælde dannelse: Dynamisk og kvantitativ analyse af Fluorescens mikroskopi
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

In Vitro Canine neutrofile ekstracellulære fælde dannelse: Dynamisk og kvantitativ analyse af Fluorescens mikroskopi

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8,539 Views

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09:45 min

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August 24, 2018

DOI:

10.3791/58083-v

DOI:
10.3791/58083-v

•

09:45 min
August 24, 2018

•

4 Views
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1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,University of California, Davis

Summary

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Vi beskriver metoder til at isolere canine neutrofiler fra fuldblod og visualisere netto dannelse i live neutrofiler ved hjælp af Fluorescens mikroskopi. Også beskrevet er protokoller at kvantificere netto dannelse og citrullinated Histon H3 (citH3) udtryk ved hjælp af immunofluorescens mikroskopi.

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Cite this Article

Li, R. H., Tablin, F. In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy. J. Vis. Exp. (138), e58083, doi:10.3791/58083 (2018).

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