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Journal / Immunology and Infection / In Vitro Hundarnas neutrofila extracellulära…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Hundarnas neutrofila extracellulära fälla bildandet
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In Vitro Hundarnas neutrofila extracellulära fälla bildandet: Dynamiska och kvantitativ analys av fluorescensmikroskopi

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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Vi beskriva metoder för att isolera Hundarnas neutrofiler från helblod och visualisera NET bildandet i levande neutrofiler med fluorescensmikroskopi. Beskrev också är protokoll att kvantifiera NET bildandet och citrullinerade Histon H3 (citH3) uttryck med hjälp av immunofluorescens mikroskopi.

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Immunologi och infektion fråga 138 immunofluorescens mikroskopi sepsis granulocyt isolering Histon citrullinering peptidylarginine deiminase
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