Automated Behavioral Analysis of Large <em>C. elegans</em> Populations Using a Wide Field-of-view Tracking Platform

Michele Perni; Sam Casford; Francesco A. Aprile; Ellen A. Nollen; Tuomas P.J. Knowles; Michele Vendruscolo; Christopher M. Dobson

doi:10.3791/58643

Journal

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Immunology and Infection

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Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform

Automated Behavioral Analysis of Large <em>C. elegans</em> Populations Using a Wide Field-of-view Tracking Platform

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform

Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform

9,124 Views

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07:20 min

•

November 28, 2018

DOI:

10.3791/58643-v

DOI:

10.3791/58643-v

•

07:20 min

November 28, 2018

•

9106 Views

Michele Perni¹, Sam Casford¹, Francesco A. Aprile¹, Ellen A. Nollen², Tuomas P.J. Knowles¹, Michele Vendruscolo¹, Christopher M. Dobson¹

¹Centre for Misfolding Diseases, Department of Chemistry,University of Cambridge ²European Research Institute for the Biology of Aging,University Medical Centre Groningen

Summary

We describe protocols for using the wide field-of-view nematode tracking platform (WF-NTP), which enables high-throughput phenotypic characterization of large populations of Caenorhabditis elegans. These protocols can be used to characterize subtle behavioral changes in mutant strains or in response to pharmacological treatment in a highly scalable fashion.