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Journal / Biochemistry / Using the Open-Source MALDI TOF-MS IDBac Pipeline …
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Biochemistry

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JoVE Journal Biochemistry
Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
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Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Article DOI: 10.3791/59219-v • 09:29 min • May 15th, 2019
May 15th, 2019 •
1, 1,2, 1, 1, 1, 1
1Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 2Faculty of Pharmaceutical Sciences, University of Iceland

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Summary
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IDBac is an open-source mass spectrometry-based bioinformatics pipeline that integrates data from both intact protein and specialized metabolite spectra, collected on cell material scraped from bacterial colonies. The pipeline allows researchers to rapidly organize hundreds to thousands of bacterial colonies into putative taxonomic groups, and further differentiate them based on specialized metabolite production.

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Open-Source MALDI TOF-MS IDBac Pipeline Microbial Protein Analysis Specialized Metabolite Data Analysis Mass Spec Data Bacterial Isolates Identity Discrimination Environmental Function Office Source Software MALDI TOF-based Microbial Identification Strategies Specialized Metabolism Analysis Phenotype Differentiation Unknown Microorganisms Characterization Protein Profiling Small Molecule Profiling Visual Comparison Of Specialized Metabolite Production Research Topics Ecological Studies Drug Lead Discovery MALDI Data Saving IDBac's GitHub Submission Bacterial Colony Transfer Clean MALDI Plate Preparation
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