JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
趋化梯度多细胞响应的三维分析
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
Please note that all translations are automatically generated.
Click here for the English version.
趋化梯度多细胞响应的三维分析
DOI:
10.3791/59226-v
•
05:57 min
•
May 24, 2019
•
Tae-Yun Kang
,
David Ellison
,
Sung Hoon Lee
,
Andrew J. Ewald
3
,
Andre Levchenko
1
Department of Biomedical Engineering and Yale Systems Biology Institute
,
Yale University
,
2
Department of Biomedical Engineering
,
Johns Hopkins University
,
3
Center for Cell Dynamics and Department of Cell Biology
,
Johns Hopkins University
Chapters
00:04
Title
00:34
Mesofluidic Device Fabrication
02:20
Collagen Injection, 3D Imaging, and Quantification
04:20
Results: Representative Ligand Gradient Evaluation and Cellular Gradient Reponses
05:23
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们描述了一种方法来构建设备的三维培养和实验与细胞和多细胞有机体。该装置允许分析在具有明确趋化因子梯度的三维微环境中对可溶性信号的细胞反应。在检测弱噪声输入时, 有机体优于单个细胞。
Tags
3D Analysis
Multi-cellular Response
Chemoattractant Gradients
PDMS Device
Mesofluidic Device
Stereo Lithography
Vacuum Degassing
PDMS Curing
Cell Loading
Collagen Cell Suspension
Cell Culture
3D Physiological Environment
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
循环压力为生物反应器的设计
体外
主动脉心脏瓣膜的研究
创建的胶粘剂和可溶性梯度荧光显微镜成像细胞迁移
相关显微镜的动力相互作用的三维结构分析
多尺度孔梯度,聚电解质和间接监测改性金属植入物<em>在体内</em
使用粘性图案构建3D纸微流体装置
一种意志的运动反应的及时性和准确性,以振动触觉刺激评价方法
细胞迁移研究在垂直化学和氧梯度聚二甲基硅氧烷的聚碳酸酯微流体装置
Bioink Precellularization 无源混合装置打印软骨及皮肤组织类似物的应用
使用顺序 3D-3D 注册进行四维 CT 分析
通过仪器和分析工具量化动眼神经反应和调节
Read Article