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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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Cancer Research
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JoVE Journal Cancer Research
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

DOI:
10.3791/59322-v

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00:07 min

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August 23, 2019

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, , , ,
1Instituto de Investigaciones Biomédicas, CSIC-UAM, 2Ciberonc,Instituto de Salud Carlos III

Chapters

  • 00:04Title
  • 00:49Flank Inoculation and Intratumoral AntagomiR Treatment
  • 02:23Tumor Growth Analysis
  • 03:10Thyroid Orthotopic Inoculation and Systemic AntagomiR Treatment
  • 05:05Results: Representative Effects of In Vivo MicroRNA Inhibition on Tumor Growth in Mice
  • 06:42Conclusion

Summary

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This protocol describes xenograft and orthotopic mouse models of human thyroid tumorigenesis as a platform to test microRNA-based inhibitor treatments. This approach is ideal to study the function of non-coding RNAs and their potential as new therapeutic targets.

Tags

MicroRNA InhibitorThyroid CancerOrthotopic ModelIn Vivo InhibitionTumor GrowthBioluminescence ImagingAntagomiRCAL-62 Cell LineImmunodeficient Mice

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