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Immunology and Infection

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JoVE Journal Immunology and Infection
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Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Article DOI: 10.3791/59868-v • 08:03 min • July 15th, 2019
July 15th, 2019 •
1, 1
1Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine

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We present a method for isolating and cultivating primary human salivary gland-derived epithelial cells. These cells exhibit gene expression patterns consistent with them being of salivary epithelial origin and can be grown as salispheres on basement membrane matrices derived from Engelbreth-Holm-Swarm tumor cells or as monolayers on treated culture dishes.

Tags

Isolation Salivary Epithelial Cells Human Salivary Glands In Vitro Growth Salispheres Monolayers Primary Cellular Models Organoid-like Structures Biology Human Viruses Replication Salivary Gland Horizontal Transmission Salisphere-based System Cellular Models In Vivo Type Situation Mouse Primary Submandibular Gland Autoclaved Sterilized Dissecting Scissors Surgical Forceps Mince Salivary Gland Tissue Dispase/Collagenase Solution Incubate At 37 Degrees Celsius
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