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JoVE Journal
Cancer Research

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Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
 

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Article doi: 10.3791/60223
November 22nd, 2019

Summary November 22nd, 2019

Mouse prostate organoids represent a promising context to evaluate mechanisms that regulate differentiation. This paper describes an improved approach to establish prostate organoids, and introduces methods to (1) collect protein lysate from organoids, and (2) fix and stain organoids for whole-mount confocal microscopy.

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