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JoVE Journal
Bioengineering

This content is Open Access.

Handling and Assessment of Human Primary Prostate Organoid Culture
 

Handling and Assessment of Human Primary Prostate Organoid Culture

Article doi: 10.3791/59051
January 17th, 2019 Usage Statistics

Summary January 17th, 2019

Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding, culture maintenance, recovery from matrix gel, morphologic quantification, embedding and sectioning, FFPE sectioning, whole-mount staining, and application of commercial assays are described.

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