Journal
/
Cancer Research
/
Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models

DOI:
10.3791/58397-v

•

08:43 min

•

September 18, 2018

•

, , ,
1Department of Urology,SUNY Upstate Medical University, 2Department of Biochemistry and Molecular Biology,SUNY Upstate Medical University, 3Laboratory of Comparative Pathology,Memorial Sloan-Kettering Cancer Center, 4Boulder BioPATH, Inc

Chapters

  • 00:04TItle
  • 00:35Urogenital System (UGS) Dissection
  • 02:04Prostate Gross Anatomy and Individual Lobe Microdissection
  • 02:51Tissue Processing for 3D Culture
  • 04:41Cell Plating and Culture and Sphere Harvest
  • 06:38Results: Representative Mouse Prostate Lobe Analyses
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

Genetically engineered mice are useful models for investigating prostate cancer mechanisms. Here we present a protocol to identify and dissect prostate lobes from a mouse urogenital system, differentiate them based on histology, and isolate and culture the primary prostate cells in vitro as spheroids for downstream analyses.

Tags

ProstateMouse ModelLobesSpheroid CultureIn Vitro AnalysisGenetically-engineeredProstate CancerUrogenital SystemFat RemovalSeminal VesiclesDorsal LobesLateral LobesVentral LobesAnterior LobesDMEM

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation

Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

A 3D Organotypic Melanoma Spheroid Skin Model

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

A 3D Spheroid Model for Glioblastoma

Read Article