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Establishment and Analysis of Three-Dimensional (3D) Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and their Xenografts
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1Department of Urology,University of California, San Diego, 2Moores Cancer Center,University of California, San Diego, 3Department of Medicine,University of California, San Diego, 4Department of Radiation Oncology,University of California, Los Angeles, 5Department of Orthopedic Surgery,University of California, San Diego
Chapters
- 00:05Introduction
- 01:24Forming an Attached Round Dome
- 02:23Forming Floating Beads
- 03:19Organoid Processing for Histology
- 05:24Results: 3D Organoids from Patient-derived Xenograft and Primary Tumor Tissue
- 06:17Conclusion
Three-dimensional cultures of patient BMPC specimens and xenografts of bone metastatic prostate cancer maintain the functional heterogeneity of their original tumors resulting in cysts, spheroids and complex, tumor-like organoids. This manuscript provides an optimization strategy and protocol for 3D culture of heterogeneous patient derived samples and their analysis using IFC.
