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JoVE Journal
Immunology and Infection

This content is Open Access.

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
 

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Article doi: 10.3791/60743
March 2nd, 2020

Summary March 2nd, 2020

Fluorescently tagged antibiotics are powerful tools that can be used to study multiple aspects of antimicrobial resistance. This article describes the preparation of fluorescently tagged antibiotics and their application to studying antibiotic resistance in bacteria. Probes can be used to study mechanisms of bacterial resistance (e.g., efflux) by spectrophotometry, flow cytometry, and microscopy.

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