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JoVE Journal
Immunology and Infection

This content is Open Access.

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Article DOI: 10.3791/60743-v 08:23 min March 2nd, 2020
March 2nd, 2020



Fluorescently tagged antibiotics are powerful tools that can be used to study multiple aspects of antimicrobial resistance. This article describes the preparation of fluorescently tagged antibiotics and their application to studying antibiotic resistance in bacteria. Probes can be used to study mechanisms of bacterial resistance (e.g., efflux) by spectrophotometry, flow cytometry, and microscopy.



Fluorescent Antibiotic Probes Bacterial Resistance Visualization Spectrophotometry Microscopy Antibiotic Localization Efflux Click A Reaction Procedure Azide Antibiotic Fluorphore Alkyne Copper Sulfate Ascorbic Acid LCMS Analysis Antibiotic Scaffold Click Chemistry Reaction Ciprofloxacin Linezolid Trimethoprim Liquid Chromatography Mass Spectrometry
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