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Journal / Immunology and Infection / Visualization of Bacterial Resistance using…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Article DOI: 10.3791/60743-v • 08:23 min • March 2nd, 2020
March 2nd, 2020 •
1, 1, 1, 1
1Centre for Superbug Solutions, Institute for Molecular Biosciences, The University of Queensland

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Summary
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Fluorescently tagged antibiotics are powerful tools that can be used to study multiple aspects of antimicrobial resistance. This article describes the preparation of fluorescently tagged antibiotics and their application to studying antibiotic resistance in bacteria. Probes can be used to study mechanisms of bacterial resistance (e.g., efflux) by spectrophotometry, flow cytometry, and microscopy.

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Fluorescent Antibiotic Probes Bacterial Resistance Visualization Spectrophotometry Microscopy Antibiotic Localization Efflux Click A Reaction Procedure Azide Antibiotic Fluorphore Alkyne Copper Sulfate Ascorbic Acid LCMS Analysis Antibiotic Scaffold Click Chemistry Reaction Ciprofloxacin Linezolid Trimethoprim Liquid Chromatography Mass Spectrometry
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