High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Jesse Q. Zhang; Kai-Chun Chang; Leqian Liu; Zev  J. Gartner; Adam  R. Abate

doi:10.3791/61014

Journal

/

Bioengineering

/

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

7,020 Views

•

07:55 min

•

May 21, 2020

DOI:

10.3791/61014-v

DOI:

10.3791/61014-v

•

07:55 min

May 21, 2020

•

7001 Views

Jesse Q. Zhang^1,2, Kai-Chun Chang¹, Leqian Liu¹, Zev J. Gartner^3,5, Adam R. Abate^1,4,5

¹Department of Bioengineering and Therapeutic Sciences,University of California San Francisco ²University of California Berkeley-UCSF Graduate Program in Bioengineering,University of California San Francisco ³Department of Pharmaceutical Chemistry,University of California San Francisco ⁴California Institute for Quantitative Biosciences,University of California San Francisco ⁵Chan Zuckerberg Biohub

Summary

A bottleneck in the ‘design-build-test’ cycle of microbial engineering is the speed at which we can perform functional screens of strains. We describe a high-throughput method for strain screening applied to hundreds to thousands of yeast cells per experiment that utilizes droplet-based RNA sequencing.