Journal
/
/
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

7,020 Views

07:55 min

May 21, 2020

DOI:

07:55 min
May 21, 2020

7001 Views
, , , ,

Summary

Automatically generated

A bottleneck in the ‘design-build-test’ cycle of microbial engineering is the speed at which we can perform functional screens of strains. We describe a high-throughput method for strain screening applied to hundreds to thousands of yeast cells per experiment that utilizes droplet-based RNA sequencing.

Related Videos

Read Article