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JoVE Journal
Bioengineering

This content is Open Access.

JoVE Journal Bioengineering
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
 

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Article doi: 10.3791/61014
May 21st, 2020 •
1,2, 1, 1, 3,5, 1,4,5
1Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 2University of California Berkeley-UCSF Graduate Program in Bioengineering, University of California San Francisco, 3Department of Pharmaceutical Chemistry, University of California San Francisco, 4California Institute for Quantitative Biosciences, University of California San Francisco, 5Chan Zuckerberg Biohub

Summary
Automatic Translation
May 21st, 2020

A bottleneck in the ‘design-build-test’ cycle of microbial engineering is the speed at which we can perform functional screens of strains. We describe a high-throughput method for strain screening applied to hundreds to thousands of yeast cells per experiment that utilizes droplet-based RNA sequencing.

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