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JoVE Journal
Bioengineering

This content is Open Access.

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
 

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Article DOI: 10.3791/61014-v
May 21st, 2020

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Summary May 21st, 2020

A bottleneck in the ‘design-build-test’ cycle of microbial engineering is the speed at which we can perform functional screens of strains. We describe a high-throughput method for strain screening applied to hundreds to thousands of yeast cells per experiment that utilizes droplet-based RNA sequencing.

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