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Bioengineering

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JoVE Journal Bioengineering
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High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Article DOI: 10.3791/61014-v • 07:55 min • May 21st, 2020
May 21st, 2020 •
1,2, 1, 1, 3,5, 1,4,5
1Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 2University of California Berkeley-UCSF Graduate Program in Bioengineering, University of California San Francisco, 3Department of Pharmaceutical Chemistry, University of California San Francisco, 4California Institute for Quantitative Biosciences, University of California San Francisco, 5Chan Zuckerberg Biohub

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Summary
Automatic Translation

A bottleneck in the ‘design-build-test’ cycle of microbial engineering is the speed at which we can perform functional screens of strains. We describe a high-throughput method for strain screening applied to hundreds to thousands of yeast cells per experiment that utilizes droplet-based RNA sequencing.

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High Throughput Yeast Strain Phenotyping Droplet-based RNA Sequencing ICO-seq Microbes Microbial Function Genome-wide Scale Engineered Microbes Yeast Suspension Culture Cells Count Ultra-low Melting Point Agarose Syringe Filter Syringe Pump Space Heater Fluorinated Oil Ionic Fluorosurfactant Tubing Emulsion Perfluoro-octanol
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