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JoVE Journal Neuroscience
Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
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Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

Article DOI: 10.3791/61493-v • 11:04 min • September 3rd, 2020
September 3rd, 2020 •
*1,2, *1, 4, 1,2, 3,5, 3, 1,2,3
1Chronobiology of Reproduction Research Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 2Developmental Biology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 3Neuroendocrinology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 4Facultad de Ciencias, Universidad Nacional Autónoma de México, 5Department of Basic Research, Instituto Nacional de Geriatria, Ministry of Health
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes the construction of a low-cost microinjection system, its stereotaxic implantation into deep-brain structures and the procedure for timed microinjections of tetrodotoxin in awake and unrestrained rats. The goal is to reveal the participation of hypothalamic structures in the regulation of ovulation by inhibiting their neural activity.

Tags

Regulate Ovulation Discrete Areas Of The Rat Brain Tetrodotoxin Microinjections Neuronal Circuits Estrous Cycle Brain Regulation Art Fabricator Montserrat Bola Lesion And Deafferentation Stereotaxic Implantation Guide Cannulas Drug Microinjection Sodium-dependent Action Potentials Neural Activity Physiological Processes
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