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Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
 

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Article DOI: 10.3791/61579-v
September 22nd, 2020

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Summary September 22nd, 2020

In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation models.

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