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Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
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Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Article DOI: 10.3791/61728-v • 08:51 min • September 15th, 2020
September 15th, 2020 •
1, 2,3, 1,2, 1
1Centre for Biospectroscopy and School of Chemistry, Monash University, 2Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, 3Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University

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Summary
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Atomic Force Microscopy-Infrared Spectroscopy (AFM-IR) provides a powerful platform for bacterial studies, enabling to achieve nanoscale resolution. Both, mapping of subcellular changes (e.g., upon cell division) as well as comparative studies of chemical composition (e.g., arising from drug resistance) can be conducted at a single cell level in bacteria.

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Atomic Force Microscopy Infrared Spectroscopy Bacterium Chemistry Nanoscale Studies Antimicrobial Resistance AMR Drug Development AFM-IR Sub-cellular Level Molecular Targets Antimicrobials Cells Tissues Viruses Sample Preparation Bacterial Suspension
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