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Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells

Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells

DOI:
10.3791/62300-v

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07:57 min

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April 05, 2021

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, , , , ,
1Department of Cell and Developmental Biology and the Rocky Mountain Taste and Smell Center,University of Colorado Anschutz Medical Campus

Chapters

  • 00:05Introduction
  • 00:41Isolation of CVP Epithelium
  • 02:19Dissociation of CVP Epithelium
  • 04:07Plating of Lgr5-EGFP Cells and Organoid Maintenance
  • 05:41Results: Growth and Differentiation of the Lingual Organoids
  • 07:28Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presents a method for culturing and processing lingual organoids derived from taste stem cells isolated from the posterior taste papilla of adult mice.

Tags

Keywords: OrganoidsTongueTaste Stem CellsTongue CultureLingual OrganoidsMouse Taste CellsIn Vitro Taste StudiesTongue DissectionTaste Cell RegenerationEpithelium DissociationTaste Cell Development

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