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Live Imaging of Mouse Secondary Palate Fusion
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Developmental Biology
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JoVE Journal Developmental Biology
Live Imaging of Mouse Secondary Palate Fusion

Live Imaging of Mouse Secondary Palate Fusion

DOI:
10.3791/56041-v

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06:10 min

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July 27, 2017

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, ,
1Department of Cell and Tissue Biology, Program in Craniofacial Biology and Institute for Human Genetics,University of California San Francisco, 2Laboratory of Transgenic Models Diseases,Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences

Chapters

  • 00:05Title
  • 00:42Palate Explant Culture and Confocal Time-lapse Imaging
  • 03:28Results: Representative Live Palate Explant Culture Imaging
  • 05:16Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for live imaging of mouse secondary palate fusion using confocal microscopy. This protocol can be used in combination with a variety of fluorescent reporter mouse lines, and with pathway inhibitors for mechanistic insight. This protocol can be adapted for live imaging in other developmental systems.

Tags

Keywords: Live ImagingMouseSecondary Palate FusionConfocal MicroscopyCranial Facial DevelopmentPalatal ShelvesMidline Epithelia SeamEpithelial ConvergenceExplant Culture

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