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Journal / Biochemistry / En prøveforberedelsespipeline til mikrokrystaller …
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Biochemistry

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JoVE Journal Biochemistry
En prøveforberedelsespipeline til mikrokrystaller på VMXm Beamline
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En prøveforberedelsespipeline til mikrokrystaller på VMXm Beamline

Article DOI: 10.3791/62306-v • 09:00 min • June 17th, 2021
June 17th, 2021 •
1, 1,2, 1, 1,3, 1, 1, 1,4
1Diamond Light Source Ltd, Harwell Science and Innovation Campus, 2Paul Scherrer Institut, 3Central Laser Facility, Science and Technologies Facilities Council, Harwell Science and Innovation Campus, 4Rosalind Franklin Institute, Harwell Science and Innovation Campus

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Signal-til-støj-forholdet mellem data er en af de vigtigste overvejelser i udførelsen af X-ray diffraktion målinger fra mikrokrystaller. VMXm-beamline giver et støjsvagt miljø og mikrobjælke til sådanne eksperimenter. Her beskriver vi prøveforberedelsesmetoder til montering og afkøling af mikrokrystaller til VMXm og andre mikrofokus makromolekylær krystallografistrålelinjer.

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Biokemi udgave 172 Kryo-krystallografi mikrokrystallografi makromolektær krystallografi mikrokrystal elektron diffraktion.
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