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Biology
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Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
 

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Article DOI: 10.3791/62534-v 09:48 min April 22nd, 2022
April 22nd, 2022

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Summary

Cleavage under targets and tagmentation (CUT&Tag) is an efficient chromatin epigenomic profiling strategy. This protocol presents a refined CUT&Tag strategy for the profiling of histone modifications in plants.

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Tags

H3K4me3 Modification Profiling Plants Cleavage Under Targets And Tagmentation Chromatin Protein-affiliated DNA Fragments Intact Nuclei Isolation Chromatin Modifications Recognition Antibody Tethering Protein A-transposase Fusion Protein Tn5 Transposase Magnesium Ions Activation Adapters Integration DNA Preparation Polymerase Chain Reaction Enrichment Epigenomics Regulation Histone Modifications Transcription Factors Behaviors Non-coding RNAs With Recruited Proteins Temporal And Spatial Control Of Gene Expression CUT&Tag Technology Henikoff Lab Enzyme Tethering Method Chromatin Epigenomics Profiling Cotton Leaves As Experimental Materials
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