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Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells
 

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

Article DOI: 10.3791/62902-v
August 13th, 2021

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Summary August 13th, 2021

In this work, we describe a modified protocol to test mitochondrial respiratory substrate flux using recombinant perfringolysin O in combination with microplate-based respirometry. With this protocol, we show how metformin affects mitochondrial respiration of two different tumor cell lines.

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