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Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

DOI:
10.3791/63574-v

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05:35 min

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May 31, 2022

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, , ,
1Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 2GLIA Center,University of Yamanashi

Chapters

  • 00:04Introduciton
  • 01:04Preparation of iPSMG
  • 02:01Transnasal Transplantation
  • 03:43Results: Viability of iPSMG in the Cortex and the Hippocampus
  • 04:27Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presented here allows the transplantation of induced pluripotent stem cell-derived human microglia (iPSMG) into the brain via a transnasal route in immunocompetent mice. The method for the preparation and transnasal transplantation of cells and the administration of cytokine mixture for the maintenance of iPSMG is shown.

Tags

Keywords: Induced Pluripotent Stem Cell-derived MicrogliaTransnasal TransplantationImmunocompetent MiceCSF1 Receptor Antagonist PLX5622Non-invasiveNeurodegenerative DiseasesHyaluronidaseCell Transplantation

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